Technician for the Telomeres and Telomerase Group. @cnio:_cancer

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The Telomeres and Telomerase Group, within the Molecular Oncology Programme, is seeking a Technician to participate in different projects in the field of Telomeres and Telomerase.

Se Requiere

- University Degree in the field of biology. Currently being registered as a PhD student at UAM. Fellowship obtained at the PhD level.
- Category C (former B) and D (former C) Animal Experimentation certificates. - 9 years of laboratory work experience in Molecular Biology. - 2 first author publications one of which in the Telomere field. - Experience with research in countries outside of native country. - Participation in international conferences in the Telomere field. - Research experience in telomerase and telomeres field. Research experience with shelterin proteins. - Technical skills: Working with mouse models: handling, euthanasia, extracting organs, anesthesia, intratracheal inoculation with virus, bone marrow extraction and bone marrow transplantation experiments, gene therapy using adeno-associated virus vectors(rAAV), neuromuscular coordination studies: tightrope and rotarod tests, memory study: object recognition assay, GTT (glucose tolerance test), DEXA (dual-energy x-ray absorptiometry). Q-FISH and HT(high throughput) Q-FISH to measure telomere length; IFA (immunofluorescence assay); FACS analysis. SA(senescence-associated) âGal Staining. Western Blotting. Total mRNA purification from cell cultures and tissue samples. PCR, RT-qPCR. Cloning, plasmid preparation from a bacterial cultures (Mini/Midi/MaxiPrep). Cell culture techniques. Processing of human and mouse blood samples. PBMC isolation from mouse and human blood samples. Microscopy: Transmitted Light Microscopy; Confocal Microscopy; High-Content Confocal Imaging (Opera); Super-Resolution Microscopy (STED). Computer knowledge: MS Office (Word, Excel, Access, Powerpoint), Adobe Photoshop, Sigma Plot, PubMed search, ImageQuant, QuantityOne, BLAST, VectorNTI, Leica Application Suite Advanced Fluorescence (LAS AF) software platform, Definiens Developer XD. - Fluent in English and Spanish. 

Se Ofrece

- The opportunity to be part of a Cancer Research Centre of excellence.
- Competitive salary. - An energetic working environment. - Contract linked to a project.

Procedencia CNIO
País España
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